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The 3D viewer supports almost all microscopic observation methods – bright field, dark field, incident light, fluorescence – at nearly any magnification.īecause DSLRs and system cameras have highly sophisticated camera sensors with up to 40 MP resolution and a huge dynamic range, it is possible to produce a highly accurate 3D dataset. The object is displayed in a box, where it can be rotated and observed from any angle. Helicon’s “ 3D viewer” utility, which is included into the Helicon Focus installation, is designed to visualise 3D models. The video above shows how a 3D model is created The data in the image file are then exported to create a 3D model with just one click.

Once the z-stack has been created, the rendering process can be started. The objective lenses are also available without a tripod. Thanks to its modular construction, it is extremely flexible and enables a multitude of magnification combinations (3x -> 18x), depending on which configuration of tube elements is used.

The LM macroscope has a central optical path and is thus considerably more powerful than comparable systems with a dual optical path or conventional stereomicroscopes. The image above shows an LM macroscope with Cognisys StackShot system. Achieving maximum resolution is a key aspect in the design of our devices. With our LM macroscopes and photomicroscopes, which are equipped with objective lenses that are optimised for this technique, it is possible to achieve the best possible image quality for each individual image. It takes just a few mouse clicks and minutes of your time to create the finished image, which translates into significant improvements in workflow efficiency. Because the image collection process is automated, all the images in the z-stack are captured with the same step size. The finished z-stack, which contains more than 100 separate images, is then merged into one frame using the Helicon Fokus software. These z-stacks can also be created by using a motorised focus rail ( StackShot) and the Helicon Remote software. We used a simple coin cell battery, available everywhere batteries are sold, for this process. With a specialised software, a composite image is then created, based on the sharpest regions from each of these separate photos. Over 100 separate photos are taken by gradually incrementing the focusing distance across the subject (this is the “z-stack”).

Especially at higher magnifications of 30x, where, due to physical limits, the depth of field is very low, photographers often use focus stacking to improve depth of field.

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In microscopy and macroscopy, focus stacking and the corresponding software tools are increasingly employed to extend the apparent depth of field of a photograph.

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Low budget 3D microscopy: how to create fascinating 3D transformations of a z-stack image series in microscopy and macroscopy How can I visualise surface features such as wear and tear or surface roughness? How can I capture an image of an object under the microscope that makes it look as if it’s popping out from the screen?

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